– obtained from bacterial cells where they act as an immune system, they digest foreign DNA molecules from viruses

– molecular scissors that cut DNA at a specific base-pair sequence (the recognition site)

– they scan DNA, stop at their recognition sites, then disrupt the phosphodiester bonds and the hydrogen bonds between nucleotides cutting a molecule of DNA into fragments

– blunt ends are fragment ends of a DNA molecule that are fully base paired (no overhang), resulting from cleavage by a restriction enzyme

– sticky ends fragment end of a DNA molecule with short single-stranded overhangs, resulting from cleavage by a restriction enzyme

DNA Ligase

– sticky ends can form hydrogen bonds between complementary base pairs, but the phosphodiester bonds must be reformed to stabilize the DNA molecule

– DNA ligase is an enzyme that reforms phosphodiester bonds joining DNA fragments together

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