– obtained from bacterial cells where they act as an immune system, they digest foreign DNA molecules from viruses

– molecular scissors that cut DNA at a specific base-pair sequence (the recognition site)

– they scan DNA, stop at their recognition sites, then disrupt the phosphodiester bonds and the hydrogen bonds between nucleotides cutting a molecule of DNA into fragments

– blunt ends are fragment ends of a DNA molecule that are fully base paired (no overhang), resulting from cleavage by a restriction enzyme

– sticky ends fragment end of a DNA molecule with short single-stranded overhangs, resulting from cleavage by a restriction enzyme

DNA Ligase

– sticky ends can form hydrogen bonds between complementary base pairs, but the phosphodiester bonds must be reformed to stabilize the DNA molecule

– DNA ligase is an enzyme that reforms phosphodiester bonds joining DNA fragments together

author avatar
William Anderson (Schoolworkhelper Editorial Team)
William completed his Bachelor of Science and Master of Arts in 2013. He current serves as a lecturer, tutor and freelance writer. In his spare time, he enjoys reading, walking his dog and parasailing. Article last reviewed: 2022 | St. Rosemary Institution © 2010-2024 | Creative Commons 4.0

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